Protists as mediators of complex microbial and viral associations
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Paper Summary
Key Points and Thoughts
Sampled hundreds of ciliate and testate amoeba Studied both the environment of the samples and their holobiomes Environment extract total DNA sequence all DNA assemble genomes (MAGs) identify organisms built co-occurrence networks Holobiome isolate individual cells whole genome amplification sequence amplified DNA separate sequences predict symbiotic relationships Broad Results: Single protist cells contained bacterial endosymbionts Protists co-occured with DNA viruses (found consistently) Protist-bacteria-virus interactions were found (multipartite associations)